Skip to main content
. Author manuscript; available in PMC: 2015 Oct 28.
Published in final edited form as: Nat Commun. 2015 Apr 28;6:6885. doi: 10.1038/ncomms7885

Figure 4. GAL from both the DRG and tumour promote PNI.

Figure 4

(a) Strategy to remove GAL from CM (left panel). Depletion is verified by ELISA (right panel). (b) CM was depleted of GAL by antibody and used as chemoattractant for UM-SCC-1-GALR2 cells in a modified Boyden chamber invasion assay, and less invasion is observed. (c) DRG treated with CM from UM-SCC-1-GALR2 cells have more neuritogenesis (arrows) than DRG treated with CM from UM-SCC-1-pcDNA cells (scale bar = 1mm). Neurite extensions were quantified (right panel). (d) SH-SY5Y neuroblastoma cells extend more neurites (arrows) when treated with CM from UM-SCC-1-GALR2 cells than when GAL was depleted. (For all studies n = 3 per group, scale bar = 100μm; *P < 0.05, two sample t-test, data represent mean + SD).