. Author manuscript; available in PMC: 2015 Jun 22.

Published in final edited form as: Eur J Neurosci. 2012 Nov 22;37(3):457–468. doi: 10.1111/ejn.12058

Table 1. Immunohistochemical reagents.


Fos/TH Immunohistochemistry

Fos	Serum	Normal goat serum. Pel-Freez Biologicals, Rogers, AR
	Primary	c-Fos (4): rabbit, sc-52. 1:10,000, 0.02μg IgG/ml solution, Santa Cruz Biotech, Santa Cruz, CA
	Secondary	Biotinylated goat anti-rabbit IgG (H+L). 1:500, 3μg IgG/ml solution, Vector Laboratories, Burlingame, CA
TH	Serum	Normal goat serum. Pel-Freez Biologicals, Rogers, AR
	Primary	Mouse anti-TH monoclonal antibody. 1:2,000, Millipore-Chemicon, Billerica, MA
	Secondary	Biotinylated goat anti-mouse IgG (H+L). 1:500, 3μg IgG/ml solution, Vector Laboratories, Burlingame, CA
Fos/orexin Immunohistochemistry

Fos	Serum	Normal donkey serum. Jackson ImmunoResearch Laboratories, West Grove, PA
	Primary	c-Fos (4): rabbit, sc-52. 1:10,000, 0.02μg IgG/ml solution, Santa Cruz Biotech, Santa Cruz, CA
	Secondary	Biotinylated donkey anti-rabbit IgG (H+L). 1:500, 2.4μg IgG/ml solution, Jackson ImmunoResearch, West Grove, PA
Orexin	Serum	Normal horse serum, S-2000. Vector Laboratories, Burlingame, CA
	Primary	Orexin-A (C-19): goat, sc-8070, 1:5,000, 0.04μg IgG/ml solution, Santa Cruz Biotech, Santa Cruz, CA
	Secondary	Biotinylated horse anti-goat IgG (H+L), 1:500, 3μg IgG/ml solution, Vector Laboratories, Burlingame, CA
Other reagents

Avid :Biotin Complex		Peroxidase- Vectastain ABC Kit PK-6100, Vector Laboratories, Burlingame, CA
Enzyme Substrate		Peroxidase- Vector SG Substrate Kit SK-4700, Vector Laboratories, Burlingame, CA
Enzyme Substrate		Peroxidase- 3,3′ Diaminobenzidine tetrahydrochloride, Sigma-Aldrich, St. Louis, MO