Table 2. Selection analysis of the S gene of OC43 genotypes.

Genotype	Mean dN/dSa	Siteb	amino acid	dN/dS ± S.E.c	Prd (ω >1)	Subunit/Domaine
B	0.29	131	T	2.27 ± 1.50	0.658	NTD
		192	L	1.93 ± 1.58	0.536	NTD
		263	S	2.28 ± 1.50	0.663	NTD
		421	G	2.28 ± 1.50	0.648	RBD
		627	I	2.11 ± 1.55	0.603	S1
		951	Q	2.41 ± 1.51	0.710	S2
C	0.20	1001	T	2.43 ± 1.42	0.768	S2
D	0.31	33	N	6.15 ± 1.78	0.94	NTD
		38	P	5.56 ± 2.35	0.836	NTD
		90	K	5.94 ± 2.04	0.902	NTD
		93	T	5.90 ± 2.08	0.895	NTD
		115	T	3.72 ± 3.11	0.535	NTD
		120	D	6.17 ± 1.76	0.943	NTD
		176	Y	3.85 ± 3.11	0.556	NTD
		184	K	6.35 ± 1.50	0.976	NTD
		195	L	3.80 ± 3.11	0.548	NTD
		265	L	5.45 ± 2.42	0.818	NTD
		266	D	4.93 ± 2.66	0.731	NTD
		267	I	5.08 ± 2.60	0.755	NTD
		354	S	3.95 ± 3.10	0.572	RBD
		395	I	5.81 ± 2.16	0.878	RBD
		521	Y	6.47 ± 1.27	0.999	RBD
		535	F	5.32 ± 2.49	0.796	RBD
		716	Q	3.61 ± 3.11	0.518	S2
		741	Q	3.61 ± 3.11	0.517	S2
		763	R	6.47 ± 1.26	1.000	S2
		768	G	4.77 ± 2.78	0.706	S2
		782	V	6.38 ± 1.45	0.982	S2
		813	S	3.59 ± 3.11	0.515	S2
		989	L	5.67 ± 2.34	0.855	S2
		1255	D	4.97 ± 2.64	0.737	S2
		1310	C	3.87 ± 3.11	0.559	S2
E	0.15	NA	NA	NA	NA

^adN/dS, the ratio of nonsynonymous (dN) to synonymous;

^bThe amino acid positions of genotype B were determined according to 87309 Belgium 2003 (AY903459) and 5240/07 (KF572844) for genotype C and D;

^cS.E. Standard error;

^dPr, probability;

^eNTD, N-terminal domain; RBD, receptor-binding domain; S, spike gene.