Figure 3.
High-resolution northern blot of tRNAMet species in control and patient fibroblasts. Treatment with alkali deacylates the tRNA and copper (Cu2+) treatment selectively deacylates the non-formylated species. Analysis of RNA from cells expressing a dominant negative phenotype (DN) resulting from MTFMT overexpression is shown in first two lanes (left) and from a patient clonal cell line in which the biochemical phenotype was rescued (R) in the last two lanes (right). The position of charged and uncharged tRNAs are shown. Hybridization with mt-tRNAVal was used as a loading control.