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. 2015 Apr 23;24(14):4114–4125. doi: 10.1093/hmg/ddv150

Figure 1.

Figure 1.

Mwk TRPC3 impairs Purkinje cell dendritic arborization. (A) Time course of Purkinje cell dendritic growth in organotypic slice cultures prepared from wild-type (WT) and mutant (Mwk) littermates. Cultures were fixed at the indicated days in vitro and stained with an anti-calbindin antibody to visualize dendritic morphology. Initially, Mwk dendritic arbors were indistinguishable from wild-type littermates but starting at around two weeks postnatally, Mwk Purkinje cells exhibited a significantly decreased size of their dendritic trees (P < 0.0001, ANOVA, n = 128). (B) Representative images of Purkinje cell dendritic morphology in organotypic slice cultures fixed at P9+DIV9 and immunostained for calbindin demonstrate the impaired arborization in Mwk Purkinje cells. The outlines of the dendritic arbors have been traced. (C) Size of the dendritic area in organotypic slice cultures (P9+DIV12) prepared from wild-type (WT) and mutant (Mwk) littermates, fixed and immunostained with an anti-calbindin antibody. Treatment with P/Q block significantly increased the dendritic area of the Mwk but not WT Purkinje cells (P < 0.0001, ANOVA, n = 218).