FIG. 5.

Inhalation of 1400 W restores expression of the tight junction protein ZO-1 after phosgene-induced lung injury. Mice were exposed to phosgene or filtered air, administered with 1400 W or vehicle (PBS) systemically (A) or by inhalation (B), and euthanized 24 h later. The lung tissues were analyzed for ZO-1 mRNA levels by real time qPCR (n = 4, mean ± SEM, P-value * < 0.05, *** < 0.001). Lung sections from animals exposed to filtered air, phosgene + aerosolized vehicle or phosgene + aerosolized 1400 W were stained for ZO-1 protein by immunohistochemistry and counterstained with hematoxylin (C, scale bar corresponds to 150 µm), or stained by immunofluorescence (red) and counterstained with DAPI (blue) (D, scale bar corresponds to 50 µm).