Figure 7. Temporal expression patterns of selected genes exhibiting a rhythmic periodicity in Nematostella.

Where available, expression patterns of corresponding genes from Acropora millepora are shown using the same colors with dotted lines and open symbols. Expression values were standardized to facilitate visualization of genes with different expression levels on the same axes. (A) Type I cryptochromes (NvCry1a and AmCry2, red symbols) exhibit peak expression around noon in both species. Type II cryptochrome expression (NvCry2, AmCry1, blue symbols) peaks during subjective night in Nematostella (4 am) and during the day (noon) in A. millepora. Accession numbers in Table 2. (B) Heat shock proteins and disulfide isomerase oscillate strongly both in A. millepora and Nematostella, but the timing differs between the two taxa. For brevity, the heat shock proteins are labeled as Hsp70 and Hsp90 because they are members of these large families. See text for additional discussion. Accession numbers in Table 2. (C) Two cytochrome P450 genes that are most closely related to the CYP17/20 family exhibit diel oscillations that are out of phase with one another (JGI Accession numbers: Cyp17/20-like 1: 230060, Cyp17/20-like 2: 164939).