Table 3.
Subjects Used in T-Cell Functional Analysis: Enzyme-linked Immunospot Assay
| Group Classification | Luminex Protein | Age (±2 SD) | Sex (F/M) | Number |
|---|---|---|---|---|
| Luminex | ||||
| Never culture positive for PA | IL-6/IL-8/MIP-1α | 52 ± 33 | 11/2 | 13 |
| All other proteins | 51 ± 30 | 18/6 | 24 | |
| >50% cultures positive for PA | IL-6/IL-8/MIP-1α | 64 ± 21 | 7/5 | 12 |
| All other proteins | 55 ± 28 | 4/4 | 8 | |
| ELISpot | ||||
| Never sputum culture positive for PA | 53 ± 31 | 22/9 | 31 | |
| <50% of sputum cultures positive for PA | 66 ± 17 | 11/1 | 12 | |
| >50% of sputum cultures positive for PA | 61 ± 26 | 8/6 | 14 | |
| ELISA | ||||
| Never sputum culture positive for PA | 54 ± 31 | 33/12 | 45 | |
| <50% of sputum cultures positive for PA | 64 ± 23 | 14/2 | 16 | |
| >50% of sputum cultures positive for PA | 61 ± 22 | 10/10 | 20 | |
| PCR | ||||
| Never culture positive for PA | 47 ± 32 | 6/1 | 7 | |
| >50% of sputum cultures positive for PA | 58 ± 26 | 2/2 | 4 |
Definition of abbreviation: ELISpot = enzyme-linked immunospot assay; MIP = macrophage inflammatory protein; PA = Pseudomonas aeruginosa; PCR = polymerase chain reaction.