Table 4.
OprF Peptide Relative Binding Affinity to HLA-DR Molecules
| OprF Peptide | DR1 | DR3 | DR4 | DR7 | DR9 | DR11 | DR13 | DR1501 |
|---|---|---|---|---|---|---|---|---|
| p1 | 1 | >1,060 | 3 | 2 | 10 | 50 | >309 | 4 |
| p2 | 2 | >1,060 | 3 | 4 | 3 | 11 | >309 | 3 |
| p3 | 13 | >1,060 | 350 | 9 | 23 | 756 | 2 | 94 |
| p4 | 1,134 | 224 | 10 | 964 | 420 | 75 | 3 | 173 |
| p5 | 15 | >1,060 | 249 | 1,420 | 122 | 1,500 | >309 | 3 |
| p6 | 36 | 21 | 11 | 10 | 52 | 354 | >309 | 49 |
| p7 | 1,793 | >1,060 | >2,968 | 534 | 37 | >4,297 | >309 | 81 |
| p8 | 1,120 | >1,060 | >2,968 | >5,056 | >1,642 | 537 | >309 | >1,119 |
| p9 | 15 | 533 | 2,000 | 1,412 | >1,642 | 1,333 | >309 | 1,771 |
| p10 | 18 | 447 | 753 | 91 | 42 | 129 | >309 | 8 |
| p11 | 112 | >1,060 | 67 | 67 | 39 | 548 | 200 | 0.5 |
| p12 | 8,571 | >1,060 | >2,968 | >5,056 | >1,642 | >4,297 | >309 | >1,119 |
| p13 | 11,429 | 26 | 1,333 | 355 | 345 | 1,500 | >309 | 417 |
| p14 | 11 | 23 | 283 | 49 | 13 | 707 | 2 | 67 |
| p15 | 8 | >1,060 | 5 | >5,056 | 13 | 22 | >309 | 20 |
| p16 | 2,673 | >1,060 | >2,968 | 9,412 | 574 | 149 | >309 | 1,458 |
| p17 | 4,286 | >1,060 | >2,968 | 1,765 | 548 | 4,167 | >309 | >1,119 |
| p18 | 66 | >1,060 | 460 | 127 | 57 | 420 | >309 | 123 |
| p19 | 212 | >1,060 | 4,333 | 241 | 177 | 8,333 | >309 | 1,250 |
| p20 | 28,571 | >1,060 | 6,000 | 11,765 | >1,642 | >4,297 | >309 | >1,119 |
| p21 | 20,000 | >1,060 | >2,968 | >5,056 | >1,642 | 6,667 | >309 | >1,119 |
| p22 | 3,714 | 683 | >2,968 | 7,059 | >1,642 | >4,297 | >309 | >1,119 |
| p23 | 94 | >1,060 | >2,968 | >5,056 | >1,642 | >4,297 | >309 | >1,119 |
| p24 | >11,262 | >1,060 | >2,968 | >5,056 | >1,642 | >4,297 | >309 | >1,119 |
| p25 | 2,857 | 13 | 1,467 | 1,412 | 237 | 2,083 | >309 | 833 |
| p26 | 822 | >1,060 | 258 | 1,065 | 1,200 | 335 | >309 | 70 |
| p27 | 71 | >1,060 | 103 | 80 | 85 | 866 | 169 | 4 |
| p28 | >11,262 | >1,060 | >2,968 | >5,056 | >1,642 | >4,297 | >309 | >1,119 |
| p29 | 1,225 | >1,060 | >2,968 | 1,412 | 600 | 342 | >309 | 1,042 |
| p30 | 949 | >1,060 | 467 | 142 | 63 | 1,667 | >309 | 38 |
| p31 | 90 | >1,060 | 161 | 290 | 64 | 130 | >309 | 33 |
| p32 | 35 | >1,060 | >2,968 | 183 | 31 | 391 | >309 | 15 |
| p33 | 14,286 | >1,060 | 2,667 | >5,056 | 3,600 | 2,917 | >309 | >1,119 |
| p34 | >11,262 | >1,060 | >2,968 | >5,056 | >1,642 | 7,500 | >309 | >1,119 |
Definition of abbreviations: HLA = human leukocyte antigen; OprF = outer membrane porin F.
Results are expressed as a relative binding ratio obtained by dividing the half maximal inhibitory concentration of each peptide by that of a reference peptide that binds strongly to the HLA molecule tested. Lower numbers correspond to a higher binding affinity. Numbers in bold (ratio of 20 or less) = high-affinity binding; numbers in italics (ratio 20–100) = moderate binding affinity. Each peptide:MHC combination was evaluated in two independent experiments.