Expression of microRNA (miRNA)-96 and 5-hydroxytryptamine 1B receptor (5-HT1BR) in human pulmonary artery smooth muscle cells (PASMCs). (A) Expression of miRNA-96 in human PASMCs from female and male patients with pulmonary arterial hypertension (PAH) and control patients without PAH (n = 4–6, all isolates repeated three times). The expression of 5-HT1BR messenger RNA (B) and protein (C) in human PASMCs from female and male patients with PAH and control patients without PAH (n = 4–6, all isolates repeated three times) and representative immunoblot of 5-HT1BR protein expression in human PASMCs from female and male patients and nonpatient control subjects. Data displayed as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, determined by one-way ANOVA with Tukey’s post hoc test. GAPDH = glyceraldehyde 3-phosphate dehydrogenase; RNU48 = small nuclear RNA 48; RQ = relative quantity.