Figure 3.

Validation of microRNA (miRNA-96) gene target 5-hydroxytryptamine 1B receptor (5-HT_1BR). (A) The effect of precursor (pre-) miRNA-96 transfection on the expression of miRNA-96 (n = 3). (B) The effect of pre–miRNA-96 on the luciferase activity of the psi-CHECK-2 control construct, the 5-HT_1BR construct, and mutated 5-HT_1BR construct (n = 3, all isolates repeated three times). Data displayed as mean ± SEM. **P < 0.01, ****P < 0.0001 determined by one-way ANOVA with Tukey’s post hoc test. miR = miRNA; neg cont = negative control; NTC = nontransfection control; RNU48 = small nuclear RNA 48; RQ = relative quantity.