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. 2015 Jun 15;191(12):1432–1442. doi: 10.1164/rccm.201412-2148OC

Figure 5.

Figure 5.

The effect of over-expressing microRNA (miRNA)-96 in human pulmonary artery smooth muscle cells (PASMCs) from female patients. The effect of pre–miRNA-96 transfection in human PASMCs from female patients on miRNA-96 expression (A), 5-hydroxytryptamine 1B receptor (5-HT1BR) messenger RNA (B), and 5-HT1BR protein (C) (n = 3, all isolates repeated three times). (D) The effect of overexpression of miRNA-96 on 5-HT–induced proliferation in human PASMCs from female patients with pulmonary arterial hypertension (n = 3, all isolates repeated three times). Data displayed as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, determined by one-way ANOVA with Tukey’s post hoc test. FBS = fetal bovine serum; GAPDH = glyceraldehyde 3-phosphate dehydrogenase; miR = miRNA; neg cont = negative control; RNU48 = small nuclear RNA 48; RQ = relative quantity.