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. 2015 Jun 22;10(6):e0131266. doi: 10.1371/journal.pone.0131266

Fig 6. Downregulation of PS1 expression in adult neural progenitor cells induces the expression of differentiation markers.

Fig 6

Quantitation of Western blot analysis reported as percentage of control values all normalized to actin. Western blot analysis (A) and quantification (B) of neurogenic signals in neural progenitor cell culture infected with lentiviral vectors expressing either control RL shRNA or PS1 shRNA. (Bi-iii). Expression of PS1-NTF (i), and Nestin (ii) are significantly reduced, while expression of Cyclin D1 (iii) and epidermal growth factor receptor (EGFR, iv) are slightly reduced following PS1 downregulation in protein extract of neurosphere cultures infected with lentivirus expressing PS1 shRNA. This suggests that reduced PS1 expression decreases proliferation and progenitor phase. Expression of Neurofilament-L (v) increased in neural progenitors infected with PS1 shRNA, supporting enhanced premature neuronal differentiation following PS1 downregulation in these cells. Levels of platelet derived growth factor receptor α (PDGFRα, vi) were not significantly different. Unpaired t-test with Welch’s correction, *P<0.05, **P<0.005. Error bars indicate ±SEM.