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. 2015 Jun 22;10(6):e0130063. doi: 10.1371/journal.pone.0130063

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© 2015 Hur, Mueller

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — (A) Fluorescence intensity (triangles) decays with time as a result of photodepletion. The fit (solid line) to an exponential decay function recovered an initial intensity F ₀ = 336 kcps and a depletion rate coefficient k _D = 0.026 s^-1. (B) Segmented Q-values $\hat{Q_{m}} (T_{S})$ for a segment time of T _S = 0.2 s with an average value of 0.025.