. 2015 Jun 22;10(6):e0131175. doi: 10.1371/journal.pone.0131175

Table 1. Total # of IFN-γ⁺ T cells in BAL in mice boosted at 16 weeks post-BCG (x 10³).

	4 wk post-boost		8 wk post-boost
	Infant	Adult	Infant	Adult
CD4	2.20 +/- 0.49	3.38 +/- 0.90	0.20 +/- 0.06	0.26 +/- 0.02
CD8	0.98 +/- 0.09 ^*	1.94 +/- 0.23 ^*	0.19 +/- 0.04	0.37 +/- 0.08

Infant and adult mice were immunized s.c. with BCG, and the AdHu5Ag85A booster vaccine was administered intranasally (i.n.) at 16 weeks post-BCG. The mice were sacrificed 4 or 8 weeks after boosting. Cells isolated from the BAL were stimulated with M.tb CF + crude BCG, or unstimulated as a control. Cells were stained with extracellular antibodies for T cell markers, followed by intracellular staining for IFN-γ, and analyzed by flow cytometry. Absolute numbers of IFN-γ⁺CD4⁺ and IFN-γ⁺CD8⁺ T cells were calculated (unstimulated subtracted from stimulated). Results are from one experiment per timepoint, n = 4-5/group/timepoint. Data are expressed as Mean ± SEM.

*, p < 0.05.

Table 1. Total # of IFN-γ+ T cells in BAL in mice boosted at 16 weeks post-BCG (x 103).

Table 1. Total # of IFN-γ⁺ T cells in BAL in mice boosted at 16 weeks post-BCG (x 10³).