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. 2015 Jun 22;10(6):e0131052. doi: 10.1371/journal.pone.0131052

Fig 1. Derivation of infectious CVB3 cDNA clone.

Fig 1

Using cDNA synthesized from the Wt CVB3 (Nancy) virus RNA, overlapping fragments 1 and 2 were amplified by PCR to generate a full-length cDNA clone. While RsrII and T7 RNA polymerase promoter sequences were inserted upstream of the 5’ NTR of fragment 1, the poly(A) tail (59 adenosine residues) and PacI sequences were inserted downstream to the 3’ NTR of fragment 2. The fragments were cloned into the pBR322 vector and sequenced. Vector map was derived using SimVector software.