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| Primer | Sequence |
|---|---|
| Fragment 1 | |
| RsrII T7 promoter | |
| P1 | 5’ATATATCGGACCG TTAAAACAGCCTG TGGGTTGAT3’ |
| PacI SpeI | |
| P2 | 5’ATATTTAATTAATCTTGACCCACTAGTGATTCTTTC3’ |
| Fragment 2 | |
| SpeI | |
| P3 | 5’GAAAGAATCACTAGTGGGTCAAGA3’ |
| PacI | |
| P4 | 5’TATATATTAATTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTCGCACCGAATGCGGAGAATTTA3’ |
Underlined nt, restriction enzyme sites; overlined nt, T7 promoter; bold nt, viral sequence.
