Fig 8. Antibody Responses in Mice Immunized with TMV-Multiconjugate Vaccines using Schedule I and II of Immunization.

Francisella specific total IgG, IgG1, IgG2a and IgG2b levels on day 28 in serum samples of C57BL/6 mice immunized with TMV-multiconjugate vaccine using Schedule II were determined by ELISA. The plates were coated with F. tularensis SchuS4 or LVS lysates. Serum samples obtained from naïve mice or those inoculated with TMV alone were used as controls. The data are represented as Mean ±S.D. of absorbance values measured at 450nm. The comparisons are shown with the data obtained from mice immunized with TMV-multiconjugate vaccine using schedule I (shown in Fig 6). Table shows comparison of antibody titers between groups of mice vaccinated with Schedule I and II vaccination regimens.