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. Author manuscript; available in PMC: 2015 Jun 22.
Published in final edited form as: Biotechnol Bioeng. 2013 Nov 30;111(4):829–841. doi: 10.1002/bit.25145

Figure 8.

Figure 8

Representative images for histological analysis of constructs at week 6. The BG encapsulated groups showed deeper staining for Alizarin red. The immunostaining intensity for collagen type I was greater in the TGF group. The gradient scaffolds showed more intense staining that indicated higher mineralization toward the osteogenic region. However, mineralization was observed at a few locations in the chondrogenic region of the gradient construct. C and O represent the locations of the chondrogenic and osteogenic sides on the sections of gradient scaffolds. Scale bar: 100 µm. PLGA poly(d,l-lactic-co-glycolic acid), TGF (transforming growth factor-β3), CS (chondroitin sulfate), TGF-CS (transforming growth factor-chondroitin sulfate), BMP (bone morphogenic protein-2), BG (bioactive glass), and BMP-BG (bone morphogenic protein-bioactive glass).