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. 2015 Jun 22;10(6):e0130911. doi: 10.1371/journal.pone.0130911

Fig 4. Effect of guggulsterone on HIF-1α expression.

Fig 4

After starvation for 20hr, HepG2 cells were pretreated CDCA (100μM) and/or guggulsterone (GS) (32μM) for 6 hours then exposed to 20%, 5% or 0.1% O2 for 4 hours. (A) Western analyses of HIF-1α and β-actin. (B and C) qRT-PCR analyses of SHP and HIF-1α, respectively. c, p ≤ 0.01; d, p ≤ 0.001. (D) Western analyses of HIF-1α and β-actin in HepG2 cells which were pretreated with CDCA (100μM) and/or guggulsterone (32μM) for 6 hours and exposed to 20%, 5% or 0.1% O2 for 4 hours. (E) Western analyses of HIF-1α and β-actin in HepG2 cells which were pretreated with MG132 (10μM), CDCA (100μM) andor guggulsterone (32μM) for 6 hours and exposed to 20%, 5% or 0.1% O2 for 4 hours.