Table 1. Primer sequences used to amplify specific fragments of porcine transcripts.
Gene symbol | Gene Bank accession | PCR-product (bp) | Melting point (°C) | Forward Primer sequence | Reverse Primer sequence |
---|---|---|---|---|---|
TOR3A | AK395388 | 110 | 83.7 | CAT CTT TCT TTT TCT CAG TAA CCT T | GGC TCC AAA TTT TCC ATC CT |
RABB1 | BX916033 | 68 | 82.2 | TGGCATCATCGTGGTGTATGA | CCTGCAGCCACTGCTTCA |
ALOX12 | NM_213931 | 97 | 84.7 | CAGGCTTGGTGTCGAGAGTT | AGTGGCAGAGCTGTTCCTTG |
GSTA1 | NM_214389 | 79 | 80.0 | GATTAAATAAAGCCGGCTGAGTGT | GGTCAAGCAGTTGATTGATAGCA |
C3 | NM_214009 | 85 | 83.3 | CGCCACCAACAGACTCTAACG | CCTGGAGGCCAGTCTTCAAG |
ITIH4 | NM_001001537 | 112 | 83.5 | GAGTGGTCAACAAGGGCAGT | CCTGGGTAGGTCACACCATC |
SPP1 | NM_214023 | 78 | 82.9 | GGACACGGACTCCGAGGAA | TCGGATTCATCGGAGTGATG |
TMOD3 | NM_214294 | 70 | 78.8 | CTCAACAGGGACCTCGAACC | CGCCTCTTGCGAACTAAGTC |