Table 3. Indirect calorimetry measurements of HFpu and HFs mice in week 5 and week 20.

	Week 5		Week 20
	HFpu	HFs	HFpu	HFs	sign.
Respiratory exchange ratio	0.832 ± 0.008	0.812 ± 0.011	0.836 ± 0.007	0.829 ± 0.008	n.s.
Energy expenditure (J s-1)	0.583 ± 0.008	0.575 ± 0.009	0.684 ± 0.018	0.678 ± 0.013	§§§§
Activity (total beam breaks (x1000))	36.4 ± 2.94	26.5 ± 2.03	25.8 ± 2.89	19.7 ± 1.37	§§§, ŦŦ
% of activity in the DP	70.0 ± 3.46	67.1 ± 1.57	64.9 ± 3.57	66.5 ± 1.44	n.s.
Feed intake (g)	2.77 ± 0.10	2.61 ± 0.24	3.14 ± 0.13	2.95 ± 0.22	n.s.
% of FI in the DP	70.4 ± 2.12	66.1 ± 5.74	63.8 ± 3.30	68.5 ± 2.94	n.s.
Water intake (ml)	1.74 ± 0.16	1.45 ± 0.20	2.25 ± 0.21	1.84 ± 0.14	§§

Data are expressed as mean ± SEM (n = 11 for HFpu and n = 12 for HFs). Indirect calorimetry measurements during normal, free-feeding conditions were performed after 5 and after 20 weeks of feeding the HFpu or HFs diet. Data were recorded and averaged over 24 hours. Feed intake (FI) and physical activity are also expressed as the percentage in the dark phase (DP) to give insight into the diurnal pattern.

§ indicates a significant effect of time (§§ P<0.01, §§§ P<0.001 and §§§§ P<0.0001).

Ŧ indicates a significant effect of the diet (ŦŦ P<0.01).

n.s. non-significant.