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Proteinuria was assessed before sacrifice. The urine diluted 1:10 was examined and graded with a score of 0 (<30mg/dL); 1 (30-99mg/dL); 2 (100-299mg/dL); or 3 (300-999mg/dL). Serum samples were assayed by ELISA using purified human RRP8 or TNP1 protein. RRP8- or TNP1-mice; C57BL/6 mice injected repeatedly with RRP8 or TNP1, respectively. normal; normal C57BL/6 mice.
