Table 1. Screening of 17 autoantigens associated with LN using a biotinylated human autoantigen library.
| Gene | Protein name | Luminescence signal | Molecular mass (kDa) | Expected isoelectric point | |
|---|---|---|---|---|---|
| patient A | patient B | ||||
| RRP8 | Ribosomal RNA-processing protein 8 | 37.94 | 7.88 | 50.7 | 9.87 |
| GABARAPL2 | Gamma-aminobutyric acid receptor-associated protein-like 2 | 25.93 | 2.23 | 13.7 | 8.22 |
| PRM2 | Protamine-2 | 17.44 | 1.27 | 13.1 | 12.08 |
| TNP1 | Spermatid nuclear transition protein 1 | 15.17 | 1.17 | 6.4 | 12.33 |
| USP10 | Ubiquitin carboxyl-terminal hydrolase 10 | 11.97 | 8.10 | 87.1 | 5.06 |
| FKBP4 | Peptidyl-prolyl cis-trans isomerase FKBP4 | 10.71 | 2.71 | 51.8 | 5.22 |
| CLIC5 | Chloride intracellular channel protein 5 | 8.47 | 1.78 | 46.5 | 4.55 |
| UBL5 | Ubiquitin-like protein 5 | 8.15 | 1.16 | 8.5 | 8.50 |
| TAF11 | Transcription initiation factor TFIID subunit 11 | 7.78 | 5.22 | 23.3 | 4.61 |
| JSRP1 | Junctional sarcoplasmic reticulum protein 1 | 7.78 | 5.04 | 36.3 | 9.89 |
| SAMHD1 | Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 | 7.02 | 3.44 | 72.2 | 6.86 |
| ADAP2 | Arf-GAP with dual PH domain-containing protein 2 | 5.15 | 1.42 | 44.3 | 9.71 |
| TRIP10 | Cdc42-interacting protein 4 | 5.05 | 1.37 | 68.4 | 5.49 |
| ETS2 | Protein C-ets-2 | 4.86 | 1.59 | 53.0 | 4.76 |
| PEX26 | Peroxisome assembly protein 26 | 4.53 | 1.68 | 33.9 | 6.09 |
| NHP2L1 | NHP2-like protein 1 | 4.19 | 2.58 | 14.2 | 8.41 |
| PRM1 | Sperm protamine P1 | 3.64 | 1.11 | 6.8 | 12.25 |
Luminescence signal was calculated as the signals/noise ratio. Expected isoelectric point was estimated using an online calculator (http://isoelectric.ovh.org/).