Fig 5. Stressed mice (sAL) did not display a similar gene expression pattern to CR treatment.

qPCR analysis of the expression levels of Per2, Leptr, Myh7, Col1a1, Apln and Myh6 in AL, sAL and CR groups following one week of dietary regimens. No changes in gene expression were detected in AL and sAL control groups indicating that stress due to sleep disruption in sAL mice has no effect on the expression of CR-responsive genes. mRNA levels were normalized to the housekeeping gene Gapdh.