Fig 4. Adoptive OT-I T cell transfer.

(A), Dose response of 5T33-OVA tumor cells to adoptive transfer of OT-I CD8+ T cells treatment. Animals were injected subcutaneously with 2x10⁶ tumor cells and received the indicated doses of OT-I CD8⁺ T cells (n = 10 mice per group). Tumor volume was determined by using a caliper. Data points represent mean ± SD of 10 measures. *** p<0,001 as determined by two-way ANOVA and Bonferonni post-tests. (B), Tumors were explanted and single cell suspensions were prepared by grinding tumors in a tissu grinder. Cells were stained with monoclonal anti-CD8b PE and anti-CD45.1 FITC. Histograms represent the percentage of CD45.1⁺ in CD8⁺ cells.