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. Author manuscript; available in PMC: 2015 Jun 22.
Published in final edited form as: Methods Mol Biol. 2009;486:151–165. doi: 10.1007/978-1-60327-545-3_11

Figure 1.

Figure 1

Fluorescence compensation to correct for red fluorescence spillover into the green fluorescence detection channel. A. Uncompensated fluorescence data. B. Fluorescence profile after compensation. Approximately 3% of the signal detected in the red FL4 channel for each event was subtracted from the corresponding signal detected in the green FL1 channel. The plots represent combined data from all 384 wells.