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. Author manuscript; available in PMC: 2015 Jun 22.
Published in final edited form as: Methods Mol Biol. 2009;486:151–165. doi: 10.1007/978-1-60327-545-3_11

Figure 2.

Figure 2

Electronic gates required for analysis. A. A light scatter gate (gate 1) is constructed to exclude debris and non-viable cells from the analysis. B. Two additional gates are constructed on the basis of the color coding signals in the red fluorescence channel. One encloses red fluorescent U937/FPR cells (gate 2) and the other encloses unstained RBL/FPRL1 cells (gate 3). The plots represent combined data from all 384 wells.