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. Author manuscript; available in PMC: 2015 Jun 22.
Published in final edited form as: Methods Mol Biol. 2009;486:151–165. doi: 10.1007/978-1-60327-545-3_11

Figure 4.

Figure 4

Repetitive fluorescence intensity pattern provides supplementary sample indexing information. Illustrated are green fluorescence data from the first 96 wells of a 384-well plate. Wells are sampled from left-to right (columns 1 to 24) one row at a time, starting with the top row (row A) and moving down. This results in a repetitive pattern of low median fluorescence intensity (MFI) signals marking the 2nd and 23rd wells of each row, the locations of the positive control samples. This information facilitates identification of a row in which an indexing error has occurred.