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. 2015 Apr 7;8(4):644–658. doi: 10.1111/1751-7915.12274

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© 2015 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Fig 7 — Effect of vesicle-containing droplets on A. proliferans.A–C. Conidia of A. proliferans were incubated 4 h in liquid medium. Then, an aliquot (20 μl) of vesicles containing droplets (see Fig. A and C) was added to a culture aliquot (500 μl), and incubation continued for additional 3 h incubated for 2 h at 30°C. (A) or for 8 h (B, C). All samples were inspected microscopically by phase contrast as well as for undecylprodigiosin-derived red florescence, and are shown in a merged fashion. Vesicle-like particles that are red fluorescent () are marked. Regions from the hyphae (B) with high red intracellular fluorescence (*1, or that had developed blown up (*2), or strongly deformed (*3) areas are presented in an enlarged fashion (b¹, b² and b³). Bars 10 μm.D–F. Controls were without the addition of droplets, and inspected as outlined above. Bars 10 μm.

Inline graphic — Effect of vesicle-containing droplets on A. proliferans.A–C. Conidia of A. proliferans were incubated 4 h in liquid medium. Then, an aliquot (20 μl) of vesicles containing droplets (see Fig. A and C) was added to a culture aliquot (500 μl), and incubation continued for additional 3 h incubated for 2 h at 30°C. (A) or for 8 h (B, C). All samples were inspected microscopically by phase contrast as well as for undecylprodigiosin-derived red florescence, and are shown in a merged fashion. Vesicle-like particles that are red fluorescent () are marked. Regions from the hyphae (B) with high red intracellular fluorescence (*1, or that had developed blown up (*2), or strongly deformed (*3) areas are presented in an enlarged fashion (b¹, b² and b³). Bars 10 μm.D–F. Controls were without the addition of droplets, and inspected as outlined above. Bars 10 μm.