Fig 2.

Putative operons associated with transaminases identified in this study. A) Comparison of the genes involved in the metabolism of arginine for Pseudomonas sp. strain AAC, Pseudomonas aeruginosa PA01 and E. coli strain K-12. The transaminase of interest is shown in green. AotQ – Arginine/ornithine transport protein; AotM – Arginine/ornithine transport protein; AotP – Arginine/ornithine transport protein; ArgR -Transcriptional regulator; KES24870 / AruC– acetylornithine aminotransferase; AruF – Arginine/ornithine succinyltransferase alpha subunit; AruG – Arginine/ornithine succinyltransferase beta/all subunit; AruD – N-succinylglutamate-5-semialdehyde dehydrogenase; AstC – Succinylornithine transaminase; AstA – Arginine-N-succinyltransferase; AstD – N-succinylglutamate-5-semialdehyde dehydrogenase; AstB – N-succinylarginine dihydrolase; AstE – Succinylglutamate desuccinylase. B) The mobile element containing taurine-utilization proteins. KES22989 – Taurine–pyruvate aminotransferase; TupA – ABC Taurine transporter. C) Genes involved in pyruvate-dependent 4-aminobutyrate metabolism. KES23360 – Uncharacterized protein; GabD – Succinate semialdehyde dehydrogenase. D) Genes involved in polyamine (putrescine) transport production. KES24511 – Putrescine aminotransferase; SpuE – Polyamine transport protein; SpuD – Putrescine ABC transport protein; PotG – Polyamine transport protein; PotH – Polyamine transport protein; PotI – Polyamine transport protein. E) A second polyamine (cadaverine) transport domain, responsible for the utilization of cadaverine. CupA – Putative polyamine export protein; CupB – Putative polyamine export protein; CupC – Putative cadaverine utilization protein; KES22976 – 4-aminobutyrate aminotransferase; CupE – Putative dehydrogenase. F) GabT homologue and accompanying dehydrogenase, GabD, within Pseudomonas sp. strain AAC. KES23551 – 4-aminobutyrate aminotransferase; GabD – Succinate-semialdehyde dehydrogenase.