Table 1.
TAs identified in Pseudomonas sp. strain AAC
| Protein,a proposed functionb | Protein size (kDa)c | % Identity to GabT | Expression conditions | Activity |
|---|---|---|---|---|
| KES22976, 4-aminobutyrate aminotransferase | 44.6 | 74 | Rosetta 2-DE3, 15°C | Cadaverine |
| KES23551, 4-aminobutyrate aminotransferase | 45.2 | 73 | BL21-DE3, 15°C | 4-Aminobutyrate |
| KES24870, acetylornithine aminotransferase | 43.5 | 31 | BL21-DE3, 37°C | N-Acetyl-L-ornithine |
| KES25161, hypothetical protein | 47.5 | 30 | BL21-DE3, 37°C | β-amino acid |
| KES22989, taurine–pyruvate aminotransferase | 60.5 | 30 | Failed | |
| KES21039, 2,4-diaminobutyrate 4-aminotransferase | 50.4 | 30 | Rosetta 2-DE3, 15°C | β-alanine/aminoisobutyrate |
| KES23360, hypothetical protein | 49.3 | 27 | BL21-DE3, 15°C | Cadaverine |
| KES21385, alanine–glyoxylate aminotransferase | 47.7 | 27 | Rosetta 2-DE3, 15°C | N.D. |
| KES20403, glutamate-1-semialdehyde 2,1-aminomutase | 45.4 | 26 | Failed | |
| KES23973, adenosylmethionine-8-amino-7-oxononanoate aminotransferase | 54.2 | 25 | BL21-DE3, 37°C | N.D. |
| KES24511, aminotransferase | 50.2 | 23 | Rosetta 2-DE3, 15°C | Putrescine |
| KES23458, beta-alanine–pyruvate aminotransferase | 48.1 | 19 | BL21-DE3, 37°C | β-alanine |
| KES21511, glutamate-1-semialdehyde aminotransferase | 44.3 | 18 | Rosetta 2-DE3, 15°C | 3-Aminocyclohexanoate |
| KES22518, hypothetical protein | 42.2 | 4 | Failed |
a
Accession numbers corresponding to GenBank assembly.
b
Putative assignments for each protein predicted by BLAST analysis in UniProt database.
c
Protein size (native, untagged peptide sequence). % identity to GabT from E. coli BL21-DE3 (scoring was completed using Clustalo) is detailed. Optimal experimental conditions for protein overexpression in E. coli and the substrate with which the greatest activity for each protein was observed are also reported.
N.D., activity not determined.