Table 2.
Endophytes regrown from glycerol stock
| Seed endophytes A. thaliana | Total number | 869 | 869+ Extract | 284 | 284+ Extract |
|---|---|---|---|---|---|
| Bacillus | 4 | 4 | 4 | 0 | 2 |
| Microbacterium | 5 | 5 | 5 | 0 | 4 |
| Paenibacillus | 6 | 6 | 6 | 0 | 5 |
| Staphylococcus | 5 | 5 | 5 | 0 | 2 |
| Stem endophytes A. thaliana | Total number | 869 | 869+ Extract | 284 | 284+ Extract |
|---|---|---|---|---|---|
| Azospirillum | 1 | 0 | 1 | 0 | 0 |
| Eubacterium | 1 | 1 | 1 | 0 | 0 |
| Fluviicola | 3 | 3 | 3 | 0 | 0 |
| Kaistia | 1 | 0 | 1 | 0 | 0 |
| Nevskia | 1 | 0 | 0 | 0 | 0 |
| Paenibacillus | 1 | 1 | 1 | 0 | 0 |
| Pedobacter | 1 | 1 | 1 | 0 | 1 |
| Pelomonas | 2 | 0 | 2 | 0 | 0 |
| Pseudomonas | 1 | 0 | 0 | 0 | 0 |
| Sediminibacterium | 2 | 1 | 2 | 0 | 0 |
| Sphingomonas | 2 | 0 | 2 | 0 | 0 |
| Staphylococcus | 2 | 1 | 2 | 0 | 2 |
| Variovorax | 2 | 1 | 2 | 0 | 2 |
| Leaf endophytes A. thaliana | Total number | 869 | 869+ Extract | 284 | 284+ Extract |
|---|---|---|---|---|---|
| Acidovorax | 3 | 3 | 3 | 0 | 2 |
| Bacillus | 2 | 1 | 2 | 0 | 0 |
| Brevundimonas | 2 | 0 | 1 | 0 | 0 |
| Exiguobacterium | 2 | 2 | 2 | 0 | 2 |
| Fluviicola | 7 | 5 | 7 | 0 | 2 |
| Microbacterium | 1 | 0 | 0 | 0 | 0 |
| Pelomonas | 20 | 2 | 17 | 0 | 3 |
| Pseudomonas | 2 | 0 | 2 | 0 | 0 |
| Rhizobium | 4 | 1 | 2 | 0 | 0 |
| Rhodococcus | 1 | 0 | 1 | 0 | 0 |
| Sediminibacterium | 2 | 0 | 2 | 0 | 0 |
| Sinorhizobium | 1 | 1 | 1 | 0 | 0 |
| Variovorax | 1 | 0 | 1 | 0 | 1 |
| Seed endophytes C. pumila | Total number | 869 | 869+ Extract | 284 | 284+ Extract |
|---|---|---|---|---|---|
| Bacillus | 3 | 3 | 3 | 1 | 2 |
| Brachyobacterium | 5 | 4 | 5 | 3 | 5 |
| Curtobacterium | 4 | 4 | 4 | 1 | 2 |
| Caulobacter | 1 | 0 | 1 | 0 | 0 |
| Microbacterium | 1 | 1 | 1 | 0 | 0 |
| Staphylococcus | 6 | 6 | 6 | 3 | 5 |
| Sphingomonas | 12 | 8 | 12 | 7 | 10 |
Endophytes were isolated from seed, stem and leaf tissue of Arabidopsis thaliana and seed tissue of Crotalaria pumila. The bacterial glycerol suspension was spread on 869 and 284 medium with and without plant extract with agar as a gelling agent. ‘Total number’ depicts the number of bacteria that is attempted to be grown from glycerol stock; the other columns depict the number of bacteria that grew on the plates.