Fig 6.

Integrations into the E. coli flagellar regions 2 and 3b abolish motility. The integrations into the investigated target sites of the flagellar region 2 [motA (motAi), motB (motBi), flhD (flhDi), flhE (flhEi), cheW (cheWi), cheY (cheYi) and cheZ (cheZi)] and 3b [fliE (fliEi), fliF (fliFi), fliG (fliGi), fliJ (fliJi), fliK (fliKi), fliL (fliLi), fliM (fliMi), fliP (fliPi)] completely abolished the motility of the engineered strains. Wt (E. coli K12 MG1655 wild type). E. coli cultures normalized to OD₆₀₀ of 1 were inoculated in the motility agar plates and the picture was taken after 5 h of incubation at 37°C.