Table 4.
Bacterial strains and plasmids
| Characteristics | Reference | |
|---|---|---|
| Strains | ||
| K12 MG1655 | E. coli wild type | Hayashi et al., 2006 |
| Ec:motAi | E. coli K12 MG1655, motA integration | This study |
| Ec:motBi | E. coli K12 MG1655, motB integration | This study |
| Ec:flhDi | E. coli K12 MG1655, flhD integration | This study |
| Ec:flhEi | E. coli K12 MG1655, flhE integration | This study |
| Ec:cheWi | E. coli K12 MG1655, cheW integration | This study |
| Ec:cheYi | E. coli K12 MG1655, cheY integration | This study |
| Ec:cheZi | E. coli K12 MG1655, cheZ integration | This study |
| Ec:fliEi | E. coli K12 MG1655 integration into fliE | This study |
| Ec:fliFi | E. coli K12 MG1655 integration into fliF | This study |
| Ec:fliGi | E. coli K12 MG1655 integration into fliG | This study |
| Ec:fliJi | E. coli K12 MG1655 integration into fliJ | This study |
| Ec:fliK | E. coli K12 MG1655 integration into fliK | This study |
| Ec:fliLi | E. coli K12 MG1655 integration into fliL | This study |
| Ec:fliMi | E. coli K12 MG1655 integration into fliM | This study |
| Ec:fliPi | E. coli K12 MG1655 integration into fliP | This study |
| Ec:fliRi | E. coli K12 MG1655 integration into fliR | This study |
| Plasmids | ||
| pCP20 | Plasmid encoding FLP recombinase | Datsenko and Wanner, 2000 |
| pKM208 | IPTG-induced Red recombinase system | Murphy and Campellone, 2003 |
| pSB1A1(GFP) | λ promoter-controlled GFP, AmpR | Juhas et al., 2014b |
| pSB1A1(mCh) | λ promoter-controlled mCherry, AmpR | This study |
| pSB1K3(FRTKr) | λ repressor, KanR | Juhas et al., 2014b |