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. 2015 Jun 21;21(23):7155–7164. doi: 10.3748/wjg.v21.i23.7155

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©The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.

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Effects of bicyclol on the fibrogenic gene expression. The mRNA expression levels were assayed by reverse transcription quantitative-polymerase chain reaction with TaqMan gene expression kits (A), Western blot analysis of α-smooth muscle actin (α-SMA) and transforming growth factor (TGF)-β1/2 expression in each bile duct ligation (BDL) rat liver (B) and normalized against Gapdh. Data are expressed as mean ± SD of 6 animals per group. ^aP < 0.05 vs Sham group; ^cP < 0.05 vs BDL group. Sham: Sham group; BDL: BDL group; BDL-Bic: Bicyclol group.