Table 2.
Structural features of the 56 M. sexta CTL domain models
| domain | Ca2+ | putative Ca2+ coordinators | motif | sugar * | putative sugar binding residues | template |
|---|---|---|---|---|---|---|
| CTL-S1 | 0 | QPD | G+ | 116,125,127,129,138,155~6 | 2OX9 | |
| S2 | 1 | 75,79,95,132,142~3 | QPD | no | no | |
| S3 | 0 | QPD | G+ | 114,117,119,121,129,146~7 | 2OX9 | |
| S4 | 0 | LPM | no | no (0.16) | ||
| S5 | 0 | --- | G | 62,91,94,111~3,117 | 1SL5 | |
| S6 | 1 | 79,83,86,107,116~7 | EPN | M+ | 104,106,132~4 | 3G83 |
| S7 | 0 | KYI | M > G | 70,104,106,108,113,126~8 /105,113,117,119,126~8,132 |
2IT6 / 3P5I | |
| S8 | 0 | -PD | MLR | 94,101,115~7 | 2GGU | |
| S9A | 0 | THV | no | no | ||
| S9B | 1 | 97,111,128 | QPD | G+ | 95,97,99,111 | 2OX9 |
| S9C | 0 | LDE | no | no | ||
| CTL-X1 | 2 | 79,83,86,104,106~7 / 106,120~1 |
APG | M | 70,71,103,105,112~3,120~1 | 1K9I |
| X2 | 0 | QPN | M > G | 104,106,108,112,118~9,126~7, 129 / 101~4,106,112,126~7 |
1K9I / 3P5G | |
| X3 | 0 | QPN | G > M | 95,97,101,105,107,116~8, 122 / 95,101,116~7,119 |
3P5I / 1SL4 | |
| ×4 | 0 | QLT | no | no (0.13) | ||
| X5A | 0 | QPN | no | no | ||
| X5B | 0 | EDL | G > M | 101,107,123~4 / 123~5 | 3P5G / 1K9I | |
| X6 | 0 | --- | no | no | ||
| IML-1A | 2 |
92,95,99,111~2 / 69,95,99,100 |
QPR | G | 89,92,94,96,99,111~2 | 2OX9 |
| 1B | 2 | 112,116,118 / 111,115,118 |
EPN | M+ | 71,109,111,113,117, 123~4,130~1,133 |
1SL4 |
| 2A | 1 | 69,73,98,103,104 | EPG | Glc > M | 95,97,103,115~7 /
58,61~2, 95,97,99,103,109~10,115~6 |
1PWB / 1K9I |
| 2B | 1 | 80,84,87,110,115,116 | EPN | G > M± | 104~7,109,115,127~8 /
73, 107,109,111,115,121~2,127~8 |
3P5G / 1K9I |
| 3A | 0 | FTE | M | 63,105,108~9,116~8 | 2IT6 | |
| 3B | 0 | QPD | M > G± | 72,106,108,110,112,118~9,124~ 5,127 / 103,106,108,112,124~5 |
1SL4 / 2OX9 | |
| 4A | 0 | YYE | M | 64,99,101,106,112~3,118~20 | 1K9I | |
| 4B | 0 | EPN | M+ | 108,110,129~31 | 2IT6 | |
| 5A | 0 | EPG | M | 95,97,103,115~7 | 1K9I | |
| 5B | 2 | 80,84,87,110,115 116 / 83,110,114,116 |
EPN | M+ = G | 109,115,127~9/107,109, 111,115,121~2,126 |
1SL6 / 1K9I |
| 6A | 0 | FSE | no | no | ||
| 6B | 0 | *PD | no | no | ||
| 7A | 0 | FSE | no | no (0.11) | ||
| 7B | 0 | RKN | G | 72,107~8,110,112,123~5,129 | 1SL5 | |
| 8A | 0 | FSE | no | 2IT6 (0.13) | ||
| 8B | 0 | APN | M | 106,108,123~5 /
106,108, 112,116,123,125,131 |
2ORJ/3G84 | |
| 9A | 1 | 95,100,113~4 | KPD | M > G | 60,93,95,97,101,113~4,116 / 90~3,95,100,106,114~5 |
2IT5/3P5G |
| 9B | 0 | QPD | G+ | 103,106,108,110,113,125~6 | 2OX9 | |
| 10A | 1 | 67,71,74,96,101,102 | EPN | NPJ > M± | 93,95,97,101,107~9,113~5
/ 93,95,101,105,113,115 |
2GGX/3G84 |
| 10B | 0 | QPD | G+ | 103,106,108,113,125~6 | 2OX9 | |
| 11A | 1 | 95,100,114 | KPD | Glc = G | 90,93,95,97,101,113,114 | 2OX9 |
| 11B | 0 | QPD | G+ | 103,106,108,110,113,125~6 | 2OX9 | |
| 12A | 0 | EPN | G > M± | 90,93,95,97,101,113~4
/ 93,95,101,105,113,115 |
2OX9 / 3G84 | |
| 12B | 0 | QPD | G+ | 103,106,108,113,125,126 | 2OX9 | |
| 13A | 0 | FPE | no | no | ||
| 13B | 2 | 82,109,115,118 /
79, 83,86,109,117,118 |
RPD | M > G | 72,106,108,117,123~4,131~2, 134 / 72,109,117,131~3,137 |
1SL4 / 1SL5 |
| 14A | 0 | FPG | no | 1K9J | ||
| 14B | 2 | 108,117,132 /
79, 83,86,109,117,118 |
RPD | M | 72,106,108,117,131,132,134 | 2IT5 |
| 15A | 0 | FPE | no | no | ||
| 15B | 1 | 79,83,86,109,118,119 | QPD | M- | 72,106,108,110,118,132~3,135 | 2IT5 |
| 16A | 0 | FPE | no | no (0.12) | ||
| 16B | 2 | 79,82,109,118~9
/ 79,83,86,109,118~9 |
QPD | G+ | 103,106,108,110,118,132~3 | |
| 17A | 0 | FPE | no | |||
| 17B | 1 | 83,109,115,118 | QPD | G+ | 103,106,108,117,131~2 | |
| 18A | 2 | 70,74,77,104,105
/ 70,74,101,104,105 |
EPK | M | 98,100,104,108,116,118 | |
| 18B | 0 | CPQ | M | 69,103,105,107,114,126~7,129 | ||
| 19A | 1 | TPD | G | 93,96,98,100,104,116~7 | ||
| 19B | 0 | EPP | M | 112,118~9,124~5,127 |
*
G, galactose; M, mannose; MLR, maltotriose; NPJ, 4-nitrophenyl 4-O-α-D-glucopyranosyl-α-D-galactopyranoside; Glc: α-D-glucose; no, C-score ≤ 0.20; when X > Y> 0.20: if a sugar (X) has a higher C-score than the expected ( Y, marked ±) based on the EPN (mannose) or QPD (galactose) motif, both types are listed. Otherwise, only the sugar with highest C-score is listed and marked “+” for consistent predictions or “-” for contradictory ones. The residues involved in both Ca coordination and sugar binding are in bold.