Table I.
Effect of the Formulation Conditions (5-FU, Butylcyanoacrylate Monomer, and Dextran-70 Concentrations) for Drug Absorption on Particle Size, 5-FU Entrapment Efficiency (%), 5-FU Loading (%), and Zeta Potential (ζ, mV) Values of the Blank (5-FU Unloaded) PBCA NPs and the 5-FU-Loaded PBCA NPs Synthesized After Drug Dissolution in the Organic Phase
| 5-FU (M) | Butylcyanoacrylate (%, w/v) | Dextran-70 (%, w/v) | Size (nm) | 5-FU entrapment efficiency (%) | 5-FU loading (%) | ζ (mV) |
|---|---|---|---|---|---|---|
| 0 | 10 | 0.5 | 75 ± 10 | – | – | −25 ± 4 |
| 10−4 | 10 | 0.5 | 66 ± 13 (75 ± 10) | 43.5 ± 4.2 (36.3 ± 2.1) | 0.057 ± 0.005 (0.047 ± 0.003) | −28 ± 2 (−26 ± 3) |
| 10−3 | 10 | 0.5 | 87 ± 10 (77 ± 13) | 64.9 ± 3.6 (52.7 ± 2.2) | 0.84 ± 0.05 (0.69 ± 0.03) | −25 ± 5 (−24 ± 2) |
| 10−2 | 10 | 0.5 | 81 ± 11 (78 ± 15) | 73.8 ± 3.4 (59.3 ± 3.6) | 9.59 ± 0.44 (7.71 ± 0.47) | −26 ± 3 (−23 ± 4) |
| 10−2 | 10 | 0 | Macroaggregates | 3.1 ± 1.3 (2.6 ± 1.2) | 0.41 ± 0.17 (0.34 ± 0.16) | −27 ± 2 (−25 ± 3) |
| 10−2 | 10 | 1 | 74 ± 15 (69 ± 11) | 72.2 ± 1.9 (58.7 ± 4.8) | 9.39 ± 0.25 (7.64 ± 0.62) | −29 ± 6 (−27 ± 4) |
| 10−2 | 10 | 2 | 64 ± 12 (84 ± 13) | 75.4 ± 3.9 (60.1 ± 2.7) | 9.81 ± 0.51 (7.82 ± 0.35) | −25 ± 1 (−23 ± 5) |
| 10−2 | 10 | 3 | 68 ± 10 (73 ± 12) | 69.9 ± 4.6 (56.7 ± 5.1) | 9.09 ± 0.59 (7.38 ± 0.66) | −26 ± 2 (−27 ± 1) |
| 10−2 | 50 | 0.5 | 84 ± 11 (76 ± 10) | 71.4 ± 2.8 (58.2 ± 3.3) | 1.86 ± 0.07 (1.51 ± 0.08) | −24 ± 3 (−26 ± 4) |
| 10−2 | 100 | 0.5 | 82 ± 13 (72 ± 14) | 73.2 ± 2.9 (59.8 ± 4.6) | 0.95 ± 0.04 (0.78 ± 0.06) | −28 ± 3 (−25 ± 2) |
Size, entrapment efficiency, and drug-loading values of 5-FU-loaded PBCA NPs obtained after drug dissolution in the aqueous media are set in italics