Table II.
Effect of the Formulation Conditions (5-FU, PCL, and Dextran-70 Concentrations) for Drug Absorption on Particle Size, 5-FU Entrapment Efficiency (%), 5-FU Loading (%), and Zeta Potential (ζ, mV) Values of the Blank (5-FU Unloaded) PCL NPs and the 5-FU-Loaded PCL NPs Synthesized After Drug Dissolution in the Organic Phase
| 5-FU (M) | PCL (%, w/v) | Dextran-70 (%, w/v) | Size (nm) | 5-FU entrapment efficiency (%) | 5-FU loading (%) | ζ (mV) |
|---|---|---|---|---|---|---|
| 0 | 2 | 0.5 | 70 ± 15 | – | – | −17 ± 2 |
| 10−4 | 2 | 0.5 | 76 ± 12 (77 ± 15) | 55.2 ± 4.6 (41.4 ± 3.9) | 0.18 ± 0.02 (0.14 ± 0.01) | −14 ± 3 (−15 ± 2) |
| 10−3 | 2 | 0.5 | 67 ± 14 (70 ± 12) | 74.6 ± 3.1 (59.2 ± 3.4) | 2.43 ± 0.11 (1.92 ± 0.11) | −14 ± 4 (−16 ± 3) |
| 10−2 | 2 | 0.5 | 79 ± 16 (65 ± 15) | 82.4 ± 4.2 (66.5 ± 3.3) | 26.8 ± 1.4 (21.6 ± 1.1) | −13 ± 2 (−17 ± 2) |
| 10−2 | 2 | 0 | Macroaggregates | 3.7 ± 1.2 (2.3 ± 1.6) | 1.21 ± 0.39 (0.75 ± 0.52) | −15 ± 3 (−14 ± 4) |
| 10−2 | 2 | 1 | 73 ± 12 (81 ± 14) | 84.2 ± 3.9 (67.2 ± 2.8) | 27.4 ± 1.3 (21.8 ± 0.9) | −12 ± 3 (−12 ± 2) |
| 10−2 | 2 | 2 | 64 ± 17 (84 ± 12) | 80.3 ± 3.2 (64.6 ± 3.1) | 26.1 ± 1.1 (21.1 ± 1.1) | −15 ± 4 (−14 ± 2) |
| 10−2 | 2 | 3 | 74 ± 19 (71 ± 16) | 78.2 ± 2.6 (61.3 ± 4.2) | 25.4 ± 0.8 (19.9 ± 1.4) | −16 ± 3 (−15 ± 3) |
| 10−2 | 10 | 0.5 | 70 ± 10 (68 ± 13) | 81.6 ± 3.7 (65.2 ± 3.2) | 5.31 ± 0.24 (4.24 ± 0.21) | −13 ± 2 (−12 ± 3) |
| 10−2 | 20 | 0.5 | 75 ± 15 (72 ± 17) | 85.1 ± 4.1 (69.4 ± 2.8) | 2.77 ± 0.13 (2.26 ± 0.09) | −15 ± 1 (−16 ± 2) |
Size, entrapment efficiency, and drug-loading values of 5-FU-loaded PCL NPs obtained after drug dissolution in the aqueous media are set in italics