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. 2015 May 1;17(4):976–987. doi: 10.1208/s12248-015-9762-4

Fig. 1.

Fig. 1

a–c Characterization of the assay reference standard-recombinant hPD1(25-167)-His protein. a SDS-PAGE analysis of the purified hPD1(25-167)-His protein expressed in HEK293 cells. Lane 1: molecular weight marker, lane 2: blank, lane 3: 1 μg of the purified hPD1(25-167)-His protein. The gel was stained with Simply Blue SafeStain. b Concentration series of hPDL-1 (0.125–2 μM) binding to captured hPD1(25-167)-His protein. c Equilibrium analysis of hPDL-1 binding to hPD1(25-167)-His yields a K D of 2.8 μM (average value over multiple surfaces)