Table I.
Characterization of hPD1(25-167)-His and hPD1(25-167)-3S-IG
| hPD1(25-167)-His | hPD1 (25-167)-3S-IG | |
|---|---|---|
| Predicted monomer M.W. | 19.0 kDa | 42.6 kDa |
| Predicted dimer M.W. | 38.0 kDa | 85.3 kDa |
| Glyco-conjugate M.W. by SEC-MALS | 33.1 kDa | 114.0 kDa |
| Protein M.W. by SEC-MALS | 20.0 kDa | 93. 0 kDa |
| %Mass as carbohydrate by SEC-MALS | 39.6% | 18.4% |
| Mass fraction recovered by SEC-MALS | 90.3% | 99.9% |
| Glyco-conjugate M.W. by MALDI-TOF | 30.9 kDa | |
| Protein M.W. by HRMS | 19.0 kDa | |
| %Mass as carbohydrate by MALDI-TOF and HRMS | 38.5% |
The molecular weight (M.W.) and carbohydrate content estimate of the purified hPD1[25-167]-His and hPD-1-3S-IG proteins were determined by SEC-MALS or MALDI-TOF as described in the Materials and Methods section