Fig. 3.

The effect of BCRP on d-luciferin bioluminescence in living mice. HEK-293 cells stably transfected with BCRP or empty vector (control) were implanted to the flanks (left and right, respectively) of immunocompromised mice. a A representative mouse showing bioluminescence imaging (BLI) acquired 30 min after i.p. administration of d-luciferin, immediately before administration of the BCRP inhibitor fumitremorgin C (FTC). b The same mouse as in a imaged 12 min after i.v. administration of FTC. c Time course of BLI signal from control and transgenic ABCG2/BCRP-expressing tumors. The BLI signal from the BCRP-transfected tumor was 70% less than the control tumor before FTC injection and increased 4-fold after FTC injection to levels comparable to the control tumor. Reprinted with permission from (106)