Fig. 3.

Oxidative DNA damage and apoptosis occurs only in cells treated with 100 µM NE. (A) H9c2 cells were treated with 2 and 100 µM of NE and cells were harvested and analyzed for oxidative DNA damage and apoptosis measuring cleaved PARP (Asp214) and phosphorylated H2AX at serine (S139) (upper panel). Fold changes in mean fluorescent intensity is shown in the lower panel. (B) H9c2 myoblasts were treated with 2 and 100 µM NE. Cells were harvested at various time points, genomic DNA was isolated and then assayed for 8-OH-dG by competitive ELISA using monoclonal antibody specific for 8-OH-dG (33). Data represented ±standard error mean of three independent experiments.