Table 1.

Plackett-Burman design to select significant variables to be optimized in the production of laccase by solid state fermentation in sugarcane bagasse.

Levels	Variables
	pH	A w a	T	CuSO4	(NH4)2SO4	KH2PO4	Asnb	YEc
			°C	μM	g/L	g/L	g/L	g/L
−1	5.0	0.993	25	0	1.5	1	0	0
+1	6.0	0.999	33	150	2.5	2	0.6	0.5

^aWater activity; ^basparagine; ^cyeast extract.