Table 5.
Results of laccase activity obtained for the central composite design experiments after 5 days of solid-state fermentation on sugarcane bagasse.
| Variables | Response | |||
|---|---|---|---|---|
| Yeast extract g/L | CuSO4 μM | Ferulic acid mM | Activity U/g | |
| 1 | 4 | 75 | 1 | 61.30 |
| 2 | 10 | 75 | 1 | 51.02 |
| 3 | 4 | 225 | 1 | 98.12 |
| 4 | 10 | 225 | 1 | 58.80 |
| 5 | 4 | 75 | 3 | 50.90 |
| 6 | 10 | 75 | 3 | 46.60 |
| 7 | 4 | 225 | 3 | 68.80 |
| 8 | 10 | 225 | 3 | 65.60 |
| 9 | 1.96 | 150 | 2 | 82.19 |
| 10 | 12.04 | 150 | 2 | 52.75 |
| 11 | 7 | 24 | 2 | 62.33 |
| 12 | 7 | 276 | 2 | 35.91 |
| 13 | 7 | 150 | 0.32 | 48.03 |
| 14 | 7 | 150 | 3.68 | 89.43 |
| 15 | 7 | 150 | 2 | 158.8 |
| 16 | 7 | 150 | 2 | 149.4 |
Note: enzyme activities in units per gram of dry substrate.