Figure 3.

Effects of HEXs on Jurkat cell proliferation. (a) Viable cell number. (b) Cell cycle analysis. Cells (1 × 10⁵/mL) were treated with HEXs-L (50 µg/mL) or MTX (2 µg/mL) or culture medium (control) for 48 h and then counted by contrast phase microscopy and processed for cell cycle analysis by flow cytometry, as described in Materials and Methods section. The results express mean ± S.D. of five independent experiments. ^∗ p < 0.05, ^∗∗ p < 0.01, and ^∗∗∗ p < 0.001, related to control, by one-way ANOVA followed by Tukey's posttest.