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. 2015 Jun 9;5:290–300. doi: 10.1016/j.redox.2015.06.002

Fig. 3.

Fig. 3

Autophagy is not responsible for the decreased level of ubiquitinated proteins in sch9Δ cells. (A) The mRNA levels of ATG8 were tested in WT cells (DBY746) transformed with empty vector (WT+vector) and SCH9 deletion mutant cells (PF102(1-1)) transformed with empty vector (sch9Δ+vector) or pRS416−SCH9 (sch9Δ+Sch9) at log phase (OD600 nm=0.5). (B) GFP-Atg8 processing is enhanced by the deletion of SCH9. WT (DBY746), atg1Δ, sch9Δ and sch9Δ/atg1Δ strains were transformed with pUG36-Ura/ATG8 and the autophagy activities were detected in log phase cells (OD600 nm=0.5). (C) WT (DBY746), atg1Δ, sch9Δ and sch9Δ/atg1Δ were harvested at OD600 nm of 0.5 and the levels of ubiquitinated protein and Sch9 were tested by western blotting with actin as the loading control. (D) The quantifications of three repeats from panel C (*P<0.05, ***P < 0.001, “ns” no significance).