Fig. 6.
Decreased protein carbonylation by alleviated intracellular oxidation in sch9Δ cells. (A) Measurements of concentration of protein carbonyl groups in WT cells (TB50a) transformed with empty vector (WT + vector), SCH9 deletion mutant cells (TS120-2d) transformed with empty vector (sch9Δ+vector) or pRS416−SCH9 (sch9Δ+Sch9) in log or stationary phase. (B) Fluorescence spectrometer analysis of DCF-stained WT (TB50a) and sch9Δ cells in log or stationary phase. (C) Microscopy of DCF-stained WT (TB50a) and sch9Δ cells in log or stationary phase. Bars: 100 µm (log phase cells were collected 12–16 h after inoculation with an OD600nm of 0.5 while stationary phase cells were collected 72 h after inoculation with an OD600 nm of 8–9. *p < 0.05, **p < 0.01).