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. 2015 Jun 23;6:186. doi: 10.3389/fphys.2015.00186

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Copyright © 2015 Maneta, Warren, Hay and Khan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of NLRP3 in MBMCs, PLBMCs, and CBMCs. (A) Expression of P2X₇ was detected by immunofluorescence in MBMCs (LH panel), CBMCs (middle panel) and PLBMCs (right hand panel) with CBMCs requiring prior incubation with LPS + BzATP before NLRP3 was detectable. (B) Flow cytometry demonstrated that costimulation with LPS + BzATP resulted in 92.4% of CBMCs showing NLRP3 expression as seen in the single parameter histogram.