Figure 5.

CBMCS and PLBMCs from laboring (L) and non-laboring (NL) women incubated with LPS and BzATP leads to increased IL-1β release. Costimulation with LPS and BzATP led to increased release of IL-1β than either LPS or BzATP alone from (A) CBMCs and (B) PLBMCs. Cells from laboring women produced significantly greater amounts of IL-1β. IL-1β release was inhibited following preincubation with the P2X₇ antagonists KN-62, oATP and the caspase -1 inhibitor YVAD-CHO following costimulation with LPS and BzATP as indicated on graphs by a “+” prefix before treatment. A combination of YVAD and oATP led to greater inhibition of IL-1β than either inhibitors alone, Both LPS and BzATP_evoked IL-1β release which was inhibited by P4 and prolactin in (A) CBMCs and (B) PLBMCs. (^*P < 0.05).